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Enhanced white spot syndrome virus (WSSV) detection sensitivity using monoclonal antibody specific to heterologously expressed VP19 envelope protein

2010-02-01

Abstract excerpt

The gene encoding the VP19 envelope protein of white spot syndrome virus (WSSV) was cloned into pMAL-C2 expression vector and transformed into the BL21 Escherichia coli strain. After induction, recombinant maltose binding protein (MBP)-VP19 fusion protein was produced, purified and electroeluted before use for immunization in Swiss mice for monoclonal antibody (MAb) production. MAbs specific to VP19 can be used to...

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Literature Corpus work
d92c735b-731d-5b53-9cc6-692beb237cc4
DOI
10.1016/j.aquaculture.2009.12.005
Open publication

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Enhanced white spot syndrome virus (WSSV) detection sensitivity using monoclonal antibody specific to heterologously expressed VP19 envelope proteinDOI 10.1016/j.aquaculture.2009.12.005
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