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Penaeus monodon nucleopolyhedrovirus detection using monoclonal antibodies specific to recombinant polyhedrin protein

2011-12-01

Abstract excerpt

Two segments of the gene encoding the polyhedrin protein of Penaeus monodon nucleopolyhedrovirus (PemoNPV) were cloned into the pTYB1 (759bp) and pGEX-6P-1 (614bp) expression vectors and then transformed into the BL21 Escherichia coli strain. After induction, a fusion of the OB-N-intein (OB-N-intein; 83.2kDa) and OB-C glutathione-S-transferase (GST-OB-C; 48.4kDa) proteins were produced. They were purified by SDS-P...

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Identifiers and source

Literature Corpus work
85219b44-9c98-569d-960f-9a0ff2728432
DOI
10.1016/j.aquaculture.2011.09.013
Open publication

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Penaeus monodon nucleopolyhedrovirus detection using monoclonal antibodies specific to recombinant polyhedrin proteinDOI 10.1016/j.aquaculture.2011.09.013
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