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Ammonium sulfate improves sensitivity and avoids false negatives of polymerase chain reaction (PCR) for scale drop disease virus (SDDV) detection

2021-04-01

Abstract excerpt

Accuracy is crucial for polymerase chain reaction (PCR) diagnostic laboratories. We noticed inconsistent PCR results or false negatives that sometimes occurred when changing commercial brand of Taq DNA polymerase enzyme. Here, we tested 6 primer sets specific for 3 important aquaculture viruses (scale drop disease virus (SDDV), tilapia lake virus (TiLV), and white spot syndrome virus (WSSV)) using 7 brands of Taq...

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Literature Corpus work
c9ab9325-c492-5020-a622-001aff4439fc
DOI
10.1007/s10499-020-00639-5
Open publication

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Ammonium sulfate improves sensitivity and avoids false negatives of polymerase chain reaction (PCR) for scale drop disease virus (SDDV) detectionDOI 10.1007/s10499-020-00639-5
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