Article

Effects of sample preservation and storage times on the detection of tilapia lake virus (TiLV) RNA in tilapia tissues

2021-02-01

Abstract excerpt

Tilapia lake virus (TiLV) is a novel orthomyxo-like virus, and has genomic RNA material that is easily degradable. Hence, proper sample storage and preservation should be applied for accurate diagnostic results. In this study, we investigated various conditions for preserving tilapia tissues for the detection of TiLV. Our results revealed that RNAlater® and deep-freezing at −20 °C are the best practices to maintai...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: bd270795-4963-5b57-a29f-c7246cc3d1ef
DOI: 10.1016/j.aquaculture.2020.736240

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Effects of sample preservation and storage times on the detection of tilapia lake virus (TiLV) RNA in tilapia tissuesDOI 10.1016/j.aquaculture.2020.736240Read

Tilapia Lake Virus (TiLV) disease: Current status of understandingSemantic proximity Read

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCRSemantic proximity Read

Rapid genotyping of tilapia lake virus (TiLV) using Nanopore sequencingSemantic proximity Read

Non-lethal sampling for Tilapia Lake Virus detection by RT-qPCR and cell cultureSemantic proximity Read

Rapid genotyping of tilapia lake virus (TiLV) using Nanopore sequencing.Semantic proximity Read

A multiplexed RT-PCR Assay for Nanopore Whole Genome Sequencing of Tilapia lake virus (TiLV)Semantic proximity Read

Rapid detection of tilapia lake virus using a one-step reverse transcription loop-mediated isothermal amplification assaySemantic proximity Read

Impact of RNA Degradation on Viral Diagnosis: An Understated but Essential Step for the Successful Establishment of a Diagnosis NetworkSemantic proximity Read

Emergence of tilapia lake virus in Thailand and an alternative semi-nested RT-PCR for detectionSemantic proximity Read

Epidemiological assessment and Motif Fingerprint-based Genomic Characterization of Tilapia Lake Virus (TiLV) infection and co-infections in ColombiaSemantic proximity Read

Molecular epidemiology of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Ghanaian cultured tilapiaSemantic proximity Read

Discovery of two novel tilapia lake virus‐like virus isolates in the transcriptomic data of guppy fish (Poecilia reticulata)Semantic proximity Read