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Development of Diagnostic Real-Time Reverse Transcription-PCR Assays for SARS-CoV-2 using Polyvinyl Alcohol (PVA) Sponge as Saliva Collection Tool

2025-11-27

Abstract excerpt

Given the pandemic of COVID-19, the diagnostic challenges using Reverse Transcription-quantitative Polymerase Chain Reaction (RT-qPCR) assays for SARS-CoV-2 are mainly concentrated in the operation, which is to timely process multiple samples and to judge false-positive/false-negative accurately. Here we suggest a unique real-time RT-PCR protocol using a polyvinyl alcohol (PVA) sponge for high-throughput screening...

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Literature Corpus work: c1d7ba1b-7d04-5378-a139-2bfb6c5d4ac8
DOI: 10.1101/2025.11.24.25340856

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