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Validation and Implementation of a Direct RT-qPCR Method for Rapid Screening of SARS-CoV-2 Infection by Using Non-Invasive Saliva Samples

2020-11-25

Abstract excerpt

Background: There is an urgent need to curb COVID-19 pandemic through early identification of asymptomatic but infectious cases. We aimed to validate and implement an optimized screening method for detection of SARS-CoV-2 RNA combining use of self-collected raw saliva samples, single-step heat-treated virus inactivation and RNA extraction, and direct RT-qPCR.<br><br>Methods: The study was conducted in Sant Joan de...

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Literature Corpus work
d4fa7f88-cfbb-58d6-912a-0edd6e348087
DOI
10.2139/ssrn.3731431
Open publication

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Validation and Implementation of a Direct RT-qPCR Method for Rapid Screening of SARS-CoV-2 Infection by Using Non-Invasive Saliva SamplesDOI 10.2139/ssrn.3731431
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