Article

Benchmarking methods for genome annotation using Nanopore direct RNA in a non-model crop plant

2025-07-15

Abstract excerpt

High-quality genome annotations are essential for transcriptomic analyses investigating plant responses to environmental stress. While Nanopore long-read direct RNA (dRNA) sequencing offers a powerful approach for improving genome annotations, studies benchmarking optimal tools for this process have primarily focused on animal models. In this study, we benchmarked five annotation tools: StringTie2, IsoQuant, Bambu...

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Literature Corpus work: a8646468-aabe-5a9a-8e52-42cca85d6faa
DOI: 10.1101/2025.07.14.664836

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