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Evaluation of an rRNA-targeted oligonucleotide probe for the detection of mosquitocidal strains of Bacillus sphaericus in soils: characterization of novel strains lacking toxin genes

1996-06-01

Abstract excerpt

Colonies of Bacillus sphaericus on primary isolation have been identified using an oligonucleotide probe targeted to a specific region of the 16S rRNA. Of 3440 colonies from soil samples from Brazil, 57 hybridized to the probe in colony blots but when purified DNA was used in slot-blots the probe was more specific and only 27 isolates hybridized. Of these, 20 strains were confirmed as members of DNA homology group...

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Identifiers and source

Literature Corpus work
a4fc264d-ef49-589d-82e9-443d88eec8bf
DOI
10.1111/j.1574-6941.1996.tb00308.x
Open publication

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Evaluation of an rRNA-targeted oligonucleotide probe for the detection of mosquitocidal strains of Bacillus sphaericus in soils: characterization of novel strains lacking toxin genesDOI 10.1111/j.1574-6941.1996.tb00308.x
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