Article

Rapid preparation of Fusarium DNA from cereals for diagnostic PCR using sonification and an extraction kit.

2002-12-01

Abstract excerpt

Several PCR methods have recently been developed for Fusarium analysis in pure cultures. To use these new techniques in mycological studies and in industrial quality control, a protocol was set up for the rapid preparation of fungal DNA from cereals. An ultrasonification probe (sonotrode) and a lysis buffer were used for mechanical lysis of mycelia from infected grains. Following ultrasonification, DNA was isolate...

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Literature Corpus work: 9d8b078c-9838-503f-a863-3e0c8715d31d
DOI: 10.1046/j.1365-3059.2002.00763.x

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