Back to search

Article

One-step reverse transcription loop-mediated isothermal amplification assay for rapid detection of melon yellow spot virus

2016-05-01

Abstract excerpt

A one-step, accelerated reverse transcription loop-mediated isothermal amplification (RT-LAMP) procedure was developed for the detection of Melon yellow spot virus (MYSV). The four primers required for accelerated RT-LAMP were designed to anneal onto a conserved region in MYSV N gene. Reaction temperature, optimal Mg²⁺ concentration, and the ratio of outer/inner primers affected reaction efficiency. MYSV RT-LAMP o...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
9b4da2ee-c6b3-5285-96bc-2bdfae0aabcf
DOI
10.1007/s10658-015-0821-6
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
One-step reverse transcription loop-mediated isothermal amplification assay for rapid detection of melon yellow spot virusDOI 10.1007/s10658-015-0821-6
Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification for Rapid Detection of West Nile VirusSemantic proximity
One-Pot Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP) for Detecting MERS-CoVSemantic proximity
Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR?Semantic proximity
Versatility of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) from diagnosis of early pathological infection to mutation detection in organisms.Semantic proximity
Development of a loop-mediated isothermal amplification assay for the rapid detection of Alongshan virusSemantic proximity
Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organismsSemantic proximity
Analysis of a Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) for yellow fever diagnosticSemantic proximity
A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1Semantic proximity
Demonstration of a Very Inexpensive, Turbidimetric, Real-Time, RT-LAMP Detection Platform Using Shrimp Laem-Singh Virus (LSNV) as a ModelSemantic proximity
Development of a multiplex loop-mediated isothermal amplification (mLAMP) method for the simultaneous detection of white spot syndrome virus and infectious hypodermal and hematopoietic necrosis virus in penaeid shrimpSemantic proximity
Detection of Middle East respiratory syndrome coronavirus using reverse transcription loop-mediated isothermal amplification (RT-LAMP)Semantic proximity
Rapid, simple detection of banana bract mosaic virus in abaca using a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assaySemantic proximity
Select a neighboring publication to make it the new centre.