Article

Evaluation and Validation of a Reproducible mNGS Workflow for Gut Microbiome: Optimizing Preservation and Automated Extraction

2026-05-29

Abstract excerpt

<title>Abstract</title> <p>Technical variability in pre-analytical and analytical steps significantly hinders the reproducibility of gut metagenomic next-generation sequencing (mNGS). To address this, we developed and validated a practical mNGS workflow. First, by evaluating three commercial preservation buffers at 37°C, we identified Buffer C as the most effective for maintaining microbiome integrity under simul...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 9b1aeeab-0d24-5c0b-876d-effde05965eb
DOI: 10.21203/rs.3.rs-9206006/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Evaluation and Validation of a Reproducible mNGS Workflow for Gut Microbiome: Optimizing Preservation and Automated ExtractionDOI 10.21203/rs.3.rs-9206006/v1Read

Optimisation of Sample Storage and DNA Extraction for Human Gut Microbiome StudiesSemantic proximity Read

Variations of gut microbiome profile under different storage conditions and preservation periods: A multi-dimensional evaluationSemantic proximity Read

Optimised human stool sample collection for multi-omic microbiota analysisSemantic proximity Read

Variations of Gut Microbiome Profile Under Different Storage Conditions and Preservation Periods: A Multi-Dimensional EvaluationSemantic proximity Read

Standardization of laboratory practices for the study of the human gut microbiomeSemantic proximity Read

The impact of storage buffer and storage conditions on fecal samples for bacteriophage infectivity and metavirome analysisSemantic proximity Read

A robust metatranscriptomic technology for population-scale studies of diet, gut microbiome, and human healthSemantic proximity Read

Accelerating Gut Microbiome Research with Robust Sample CollectionSemantic proximity Read

Optimized sampling method for fecal microbiome and metabolome preservation under room temperatureSemantic proximity Read

Optimizing storage, high-molecular weight DNA extraction and genome reconstructions from human faecal samplesSemantic proximity Read

A comprehensive automated pipeline for human microbiome sampling, 16S rRNA gene sequencing and bioinformatics processingSemantic proximity Read