Article

Evidence of off-target probe binding affecting 10x Genomics Xenium gene panels compromise accuracy of spatial transcriptomic profiling

2025-04-03

Abstract excerpt

The accuracy of spatial gene expression profiles generated by probe-based in situ spatially-resolved transcriptomic technologies depends on the specificity with which probes bind to their intended target gene. Off-target binding, defined as a probe binding to something other than the target gene, can distort a gene’s true expression profile, making probe specificity essential for reliable transcriptomics. Here, w...

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Literature Corpus work: 8aef57a9-9f41-5783-9565-66a6ebfcef2e
DOI: 10.1101/2025.03.31.646342

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