Article
Influence of fecal collection conditions and 16S rRNA gene sequencing protocols at two centers on human gut microbiota analysis
2017-08-14
Abstract excerpt
<h4>Background</h4> To optimise fecal sampling and analysis yielding reproducible microbiome data, and gain further insight into sources of its variation, we compared different collection conditions and 16S rRNA gene sequencing protocols in two centers. Fecal samples were collected on three sequential days from six healthy adults and placed in commercial collection tubes (OMNIgeneGut OMR-200) at room temperature...
Topics
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- Clostridium difficile and Clostridium perfringens research
- Diet and metabolism studies
- Dysphagia Assessment and Management
- Food Security and Health in Diverse Populations
- Gut microbiota and health
- Metabolomics and Mass Spectrometry Studies
- Molecular Biology Techniques and Applications
- Mycobacterium research and diagnosis
Identifiers and source
- Literature Corpus work
- 85b50c93-818d-510c-b160-dff51e9cb24f
- DOI
- 10.1101/175877
