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Influence of fecal collection conditions and 16S rRNA gene sequencing at two centers on human gut microbiota analysis

6 Mar 2018

Abstract excerpt

To optimise fecal sampling for reproducible analysis of the gut microbiome, we compared different methods of sample collection and sequencing of 16S rRNA genes at two centers. Samples collected from six individuals on three consecutive days were placed in commercial collection tubes (OMNIgeneGut OMR-200) or in sterile screw-top tubes in a home fridge or home freezer for 6-24 h, before transfer and storage at -80...

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PMID: 29531234
DOI: 10.1038/s41598-018-22491-7

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