Article

Reverse transcription loop‐mediated isothermal amplification assays allow the rapid detection of Listeria monocytogenes in fresh‐cut fruits and vegetables

2019-08-01

Abstract excerpt

Listeria monocytogenes (L. monocytogenes) is a major concern in human food safety. Here, the development of a reverse transcription‐loop‐mediated isothermal amplification (RT‐LAMP) method, targeting the hly A gene of L. monocytogenes, to rapidly detect this important pathogen in food is described. The RT‐LAMP conditions were optimized and the following parameters established: 60 min, 63°C, 0.2 M betaine, 8.0 mM Mg...

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Literature Corpus work: 84b32f8c-ccf3-5d01-9b97-23738665059e
DOI: 10.1111/jfs.12658

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