Article

LAMP assay coupled with CRISPR/Cas12a system for portable detection of African swine fever virus

2021-02-10

Abstract excerpt

African swine fever (ASF) is one of the most severe infectious diseases of pigs. In this study, a LAMP assay coupled with the CRISPR Cas12a system was established in one tube for the detection of the ASFV p72 gene. The single-strand DNA-fluorophore-quencher (ssDNA-FQ) reporters and CRISPR-derived RNA (crRNAs) were screened and selected for the CRISPR detection system. In combination with LAMP amplification assay,...

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Literature Corpus work: 81661f21-0458-5d65-91b0-9ac8e083cd71
DOI: 10.22541/au.161292271.12539635/v1

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