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LAMP assay coupled with CRISPR/Cas12a system for portable detection of African swine fever virus

9 Aug 2021

Abstract excerpt

African swine fever (ASF) is one of the most severe infectious diseases of pigs. In this study, a loop-mediated isothermal amplification (LAMP) assay coupled with the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system was established in one tube for the detection of the African swine fever virus (ASFV) p72 gene. The single-stranded DNA-fluorophore quencher reporter and CRISPR-derived...

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PMID: 34370390
DOI: 10.1111/tbed.14285

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