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Development and clinical application of a novel CRISPR-Cas12a based assay for the detection of African swine fever virus

14 Sept 2020

Abstract excerpt

BACKGROUND: As no treatment or effective vaccine for African swine fever virus (ASFV) is currently available, a rapid, highly sensitive diagnostic is urgently needed to curb the spread of ASFV. RESULTS: Here we designed a novel CRISPR-Cas12a based assay for ASFV detection. To detect different ASFV genotypes, 19 crRNAs were designed to target the conserved p72 gene in ASFV, and several crRNAs with high activity...

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PMID: 32928112
DOI: 10.1186/s12866-020-01966-6

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