Article

LAMP assay coupled with CRISPR/Cas12a system for portable detection of African swine fever virus

2021-05-10

Abstract excerpt

African swine fever (ASF) is one of the most severe infectious diseases of pigs. In this study, a LAMP assay coupled with the CRISPR Cas12a system was established in one tube for the detection of the ASFV p72 gene. The single-strand DNA-fluorophore-quencher (ssDNA-FQ) reporter and CRISPR-derived RNA (crRNAs) were screened and selected for the CRISPR detection system. In combination with LAMP amplification assay, t...

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Literature Corpus work: 29639085-f3f9-59ec-8b99-1f8b1adcc0b8
DOI: 10.22541/au.162066484.45389678/v1

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