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Effective DNA extraction method to improve detection of Mycobacterium avium subsp. paratuberculosis in bovine feces

2014-03-31

Abstract excerpt

Paratuberculosis caused by Mycobacterium avium subsp.paratuberculosis (MAP) has extended latent periods of infection.Due to this property, difficulties in the detection of fecal shedder have been raised.A newly designed method for DNA extraction from fecal specimens, mGITC/SC was evaluated in terms of diagnostic efficiency.The detection limit of IS900 real-time PCR was about 50 MAP (1.5 cfu) in 250 mg of feces (6...

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Literature Corpus work
7e1c0a81-4691-5bcc-a31a-8dffaea22f92
DOI
10.14405/kjvr.2014.54.1.55
Open publication

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Effective DNA extraction method to improve detection of Mycobacterium avium subsp. paratuberculosis in bovine fecesDOI 10.14405/kjvr.2014.54.1.55
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