Article

Development of a real-time PCR assay for Pseudomonas cichorii, the causal agent of midrib rot in greenhouse-grown lettuce, and its detection in irrigating water

2011-06-01

Abstract excerpt

Midrib rot is an emerging disease in greenhouse production of lettuce caused by Pseudomonas cichorii, and probably introduced through contaminated irrigation water. Concentrations of 100 CFU mL⁻¹ are enough to induce the typical midrib rot symptoms. A sensitive real-time PCR assay was developed, based on a 90-bp amplicon from the pathogenicity gene cluster hrcRST and a Taqman Minor Groove Binding probe. Specificit...

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Literature Corpus work: 7d48ab63-3e13-5abc-aa3c-e65bbf7075c7
DOI: 10.1111/j.1365-3059.2010.02388.x

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