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Detection of nodavirus in barramundi, <i>Lates calcarifer</i> (Bloch), using recombinant coat protein‐based ELISA and RT–PCR

2001-03-11

Abstract excerpt

The coat protein encoded by the nodavirus RNA2 gene originally isolated from greasy grouper, Epinephelus tauvina , was cloned, expressed as a recombinant polyhistidine‐tailed fusion protein and characterized by immunoblot analysis. The purified recombinant protein was used to develop an indirect enzyme‐linked immunosorbent assay (ELISA) to detect body exudate and plasma antibodies against the coat protein in both...

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Literature Corpus work
6ed7509f-ddfa-5e4f-8744-771f62f160c0
DOI
10.1046/j.1365-2761.2001.00270.x
Open publication

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Detection of nodavirus in barramundi, <i>Lates calcarifer</i> (Bloch), using recombinant coat protein‐based ELISA and RT–PCRDOI 10.1046/j.1365-2761.2001.00270.x
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