Rapid detection of <i>Macrobrachium rosenbergii</i> nodavirus (<i>Mr</i>NV) and extra small virus (XSV), the pathogenic agents of white tail disease of <i>Macrobrachium rosenbergii</i> (De Man), by loop‐mediated isothermal amplification

Abstract excerpt

A loop-mediated isothermal amplification (LAMP) procedure is described for rapid diagnosis of white tail disease, a viral disease caused by Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV), in the giant freshwater prawn, Macrobrachium rosenbergii. This method was more sensitive than conventional RT-PCR for detecting the two viruses. A set of four primers, two outer and two inner, were...

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