Article

Molecular Detection of Colletotrichum lindemuthianum by Duplex PCR

2008-08-01

Abstract excerpt

A duplex PCR technique was developed to detect the pathogenic fungus Colletotrichum lindemuthianum infection in the tissues of common bean. Based on the differences of 24 internal transcribed spacer, DNA sequences of Colletotrichum spp. retrieved from GeneBank database, one pair of specific primers of CY1/CY2 (CY1: 5'-CTT TGT GAA CAT ACC TAA CC-3'; CY2: 5'-GGT TTT ACG GCA GGA GTG-3'), was designed. The CY1/CY2 pri...

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Literature Corpus work: 6805e392-464e-5d56-a54d-bdfe212429f0
DOI: 10.1111/j.1439-0434.2007.01386.x

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