Spoti-find: A novel, open-source void spot assay image analysis tool

Abstract excerpt

The void spot assay (VSA) is a widely used, non-invasive method for evaluating urinary behavior in rodents, but existing analysis tools are limited in scope, throughput, or accessibility. We developed Spoti-find, a stand-alone, open-source VSA image analysis application that introduces novel, biologically meaningful metrics including void circularity, distance to paper edge, and volume-based binning into primary,...

Topics

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• Behavior, Animal
• Disease Models, Animal
• Image Processing, Computer-Assisted
• Mice
• Phenotype
• Reproducibility of Results
• Software
• Urinary Bladder

Identifiers and source

Literature Corpus work

5d50fe47-510c-5eb3-a040-cd050add4957

DOI

10.1101/2025.09.30.679176