Article
Spoti-find: a novel, open-source void spot assay image analysis tool.
American journal of physiology. Renal physiology - 1 May 2026
Hardy Cara C, Sheehan Susan, Mawe Seamus, Sebasco Nick, Ricke Will, Mahoney Matt, Korstanje Ron
Abstract excerpt
The void spot assay (VSA) is a widely used, noninvasive method for evaluating urinary behavior in rodents, but existing analysis tools are limited in scope, throughput, or accessibility. We developed Spoti-find, a stand-alone, open-source VSA image analysis application that introduces novel, biologically meaningful metrics including void circularity, distance to paper edge, and volume-based binning into primary,...
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