Article

Blocking primer improves detection of tick-borne pathogens in Ixodes scapularis (black-legged ticks) from a Lyme disease hotspot region in eastern Ontario, Canada.

2026-08-21

Abstract excerpt

Tick-borne pathogen (TBP) surveillance strategies that rely exclusively on targeted methods like PCR (PCR) or immunoblots do not benefit from strain-level sequence variation. Bacterial 16S rRNA metabarcoding offers more agnostic detection but is constrained in I. scapularis by the dominance of a maternally inherited endosymbiont, Rickettsia buchneri. Here we report the design and evaluation of three R. buchneri-sp...

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Literature Corpus work: 5802441a-cd85-5eb6-9d08-47f637cd10ef
DOI: 10.64898/2026.08.17.745364

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