Back to search

Article

Development of a qPCR assay and tremabiome deep amplicon sequencing method for differentiation of fluke species in livestock

2025-08-02

Abstract excerpt

<h4>Background</h4> Trematode parasites, or flukes, are a significant economic threat to ruminant production worldwide. Traditional diagnostic methods rely on egg sedimentation from faeces, a time-consuming methodology lacking sensitivity and specificity. This study aimed to develop and validate two diagnostic methods: firstly, qPCR for accurate identification of Fasciola spp, and secondly, tremabiome, deep ampl...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
54f3285e-e536-58fd-a127-ea5094911a30
DOI
10.1101/2025.07.31.667929
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Development of a qPCR assay and tremabiome deep amplicon sequencing method for differentiation of fluke species in livestockDOI 10.1101/2025.07.31.667929
A high throughput deep amplicon sequencing method to show the emergence and spread of <i>Calicophoron daubneyi</i> in United Kingdom cattle herdsSemantic proximity
DNA sequence analyses reveal co-occurrence of novel haplotypes of Fasciola gigantica with F. hepatica in South Africa and Zimbabwe.Semantic proximity
Genetic characterization, species differentiation and detection of Fasciola spp. by molecular approaches.Semantic proximity
Development and validation of a multilocus sequence typing scheme for <i>Fasciola hepatica</i> using next-generation deep amplicon sequencingSemantic proximity
Next-Gen Intestinal Parasite Detection: Leveraging Metataxonomics for Improved Diagnosis of Intestinal Protists and HelminthsSemantic proximity
Next-generation sequencing amplicon analysis of the genetic diversity of Eimeria populations in livestock and wildlife samples from Australia.Semantic proximity
Using deep amplicon sequencing as a molecular xenomonitoring approach for detecting filarial nematodes in biting arthropod vectorsSemantic proximity
Amplicon sequencing detects, identifies, and quantifies minority variants in mixed-species infections of Cryptosporidium parasites.Semantic proximity
Genotyping and phylogenetic analysis of Fasciola species in animals from Iraq using the ITS1 marker.Semantic proximity
Development of a PCR assay and pyrosequencing for identification of important human fish-borne trematodes and its potential use for detection in fecal specimens.Semantic proximity
Transforming Clinical Parasitology: The Role of Next-Generation Sequencing (NGS) in Diagnosing Mixed Parasitic InfectionsSemantic proximity
Transforming Clinical Parasitology: The Role of Next-Generation Sequencing (NGS) in Diagnosing Mixed Parasitic InfectionsSemantic proximity
Select a neighboring publication to make it the new centre.