Article
Development of a PCR assay and pyrosequencing for identification of important human fish-borne trematodes and its potential use for detection in fecal specimens.
Parasites & vectors - 3 Mar 2014
Tantrawatpan Chairat, Intapan Pewpan M, Thanchomnang Tongjit, Sanpool Oranuch, Janwan Penchom, Lulitanond Viraphong, Sadaow Lakkhana, Maleewong Wanchai
Abstract excerpt
BACKGROUND: Small liver and minute intestinal flukes are highly prevalent in Southeast Asia. Definitive diagnosis of parasite infection is usually achieved parasitologically by finding the fluke eggs in feces. However, their eggs are difficult to differentiate morphologically in fecal samples, even for experienced technicians. The present study developed a PCR assay coupled with DNA pyrosequencing for...
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