Article

Simple and Reliable DNA Extraction Method for the Dark Pigmented Fungus, Cercospora sojina

2010-09-01

Abstract excerpt

This study used a modified cetyltrimethylammonium bromide (CTAB) method to efficiently extract DNA from the plant pathogenic fungus Cercospora sojina. Total DNA yield obtained by this method was approximately 1 mg/g of mycelia (fresh weight), and the mean ratio of A260/A280 and A260/A230 were 2.04 and 2.1, respectively. The results of random amplified polymorphic DNA (RAPD) analysis, digestion with restriction enz...

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Literature Corpus work: 4ea036bd-52a4-5d1e-b183-93a13313d6df
DOI: 10.5423/ppj.2010.26.3.289

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