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Detection of SARS-CoV-2 RNA by multiplex RT-qPCR

2020-06-17

Abstract excerpt

The current RT-qPCR assay recommended for SARS-CoV-2 testing in the United States requires analysis of three genomic targets per sample: two viral and one host. To simplify testing and reduce the volume of required reagents, we developed a multiplex RT-qPCR assay to detect SARS-CoV-2 in a single reaction. We used existing N1, N2, and RP primer and probe sets by the CDC, but substituted fluorophores to allow multip...

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Literature Corpus work
3a6219d3-62d6-5c08-b7b5-255bf3ebd37a
DOI
10.1101/2020.06.16.155887
Open publication

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Detection of SARS-CoV-2 RNA by multiplex RT-qPCRDOI 10.1101/2020.06.16.155887
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