Article

An optimization of four SARS-CoV-2 qRT-PCR assays in a Kenyan laboratory to support the national COVID-19 rapid response teams

2022-03-04

Abstract excerpt

<h4>Background: </h4> The COVID-19 pandemic relies on real-time polymerase chain reaction (qRT-PCR) for the detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), to facilitate roll-out of patient care and infection control measures. There are several qRT-PCR assays with little evidence on their comparability. We report alterations to the developers’ recommendations to sustain the testing capa...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 353ca95a-9c77-598c-8d0e-c12770f95277
DOI: 10.12688/wellcomeopenres.16063.2

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

An optimization of four SARS-CoV-2 qRT-PCR assays in a Kenyan laboratory to support the national COVID-19 rapid response teamsDOI 10.12688/wellcomeopenres.16063.2Read

True or false: what are the factors that influence COVID-19 diagnosis by RT-qPCR?Semantic proximity Read

Evaluation of WHO listed COVID-19 qPCR primers and probe in silico with 375 SERS-CoV-2 full genome sequencesSemantic proximity Read

Direct PCR with the CDC 2019 SARS-CoV-2 assay: optimization for limited-resource settingsSemantic proximity Read

SARS-CoV-2 RNA quantification using droplet digital RT-PCRSemantic proximity Read

Comparative Analysis of Four Commercial RT-PCR Diagnostic Assay for Detection of Covid-19Semantic proximity Read

Comparison of primer-probe sets among different master mixes for laboratory screening of Severe Acute Respiratory Syndrome - Coronavirus 2 (<i>SARS-CoV-2</i>)Semantic proximity Read

Real-time RT-PCR for COVID-19 diagnosis: challenges and prospectsSemantic proximity Read

Analytical sensitivity and efficiency comparisons of SARS-COV-2 qRT-PCR primer-probe setsSemantic proximity Read

How to choose the right real-time RT-PCR primer sets for the SARS-CoV-2 genome detection? Semantic proximity Read

A one-step, one-tube real-time RT-PCR based assay with an automated analysis for detection of SARS-CoV-2Semantic proximity Read

Optimization of primer sets and detection protocols for SARS-CoV-2 of coronavirus disease 2019 (COVID-19) using PCR and real-time PCRSemantic proximity Read

A Method to evaluate analytical sensitivity of qRT-PCR kits for SARS-CoV-2 detectionSemantic proximity Read