Article

Detecting RNA Viruses in Cattle: Effects of Sequencing Depth and Sequence References in Metatranscriptomics

2025-07-21

Abstract excerpt

<title>Abstract</title> <p>In this study, the impact of sequencing depth and reference genome selection is shown to influence the detection of five bovine respiratory RNA viruses in metatranscriptomes from clinical cattle samples. Metatranscriptomes were generated from pooled samples and subsampled to 20 million, 10 million, and 1 million reads. We assessed the correlation between qRT-PCR cycle threshold (Ct) val...

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Literature Corpus work: 22746434-25d7-56b8-9ec6-8df15ed195ae
DOI: 10.21203/rs.3.rs-7071637/v1

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