Article

Development of pseudotyped retroviral system for effective gene transfer and expression in penaeid shrimp cells

2017-01-01

Abstract excerpt

The pantropic retroviral expression system has been widely used to stably introduce foreign genes into the genome of dividing cells from non-mammalian systems. However, we found that the current commercial retrovirus expression system did not work in inactively dividing primary shrimp cells. To overcome this, we successfully developed a triple-pseudotyped retroviral expression system that can be used for effective...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 1f99147f-a737-5a89-bfe6-335a267ce247
DOI: 10.1016/j.aquaculture.2016.04.014

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Development of pseudotyped retroviral system for effective gene transfer and expression in penaeid shrimp cellsDOI 10.1016/j.aquaculture.2016.04.014Read

Development of a VP28-pseudotyped baculovirus expression system for efficient gene transfer in penaeid shrimpsSemantic proximity Read

Virus-like gene transfer into cells mediated by polyoma virus pseudocapsidsSemantic proximity Read

High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and MiceSemantic proximity Read

Motif mutations in pseudoknot stem I upstream of start codon in Senecavirus A genome: Impacts on activity of viral IRES and on rescue of recombinant virus.Semantic proximity Read

Cloning and Activity Analysis of Promoter of RanGTPase Gene from Shrimp, Marsupenaeus japonicusSemantic proximity Read

White Spot Syndrome Virus Annexes a Shrimp STAT To Enhance Expression of the Immediate-Early Gene <i>ie1</i>Semantic proximity Read

Constitutive expression of thymidylate synthase from LCDV-C induces a transformed phenotype in fish cells.Semantic proximity Read

Investigation of essential cell cycle regulator genes as candidates for immortalized shrimp cell line establishment based on the effect of in vitro culturing on gene expression of shrimp primary cellsSemantic proximity Read

Synonymous SNPs of viral genes facilitate virus to escape host antiviral RNAi immunitySemantic proximity Read

Antisense RNA mediated protection from white spot syndrome virus (WSSV) infection in Pacific white shrimp Litopenaeus vannameiSemantic proximity Read

Cell lines from the Egyptian fruit bat are permissive for modified vaccinia AnkaraSemantic proximity Read

Evaluation of methods for DNA delivery into shrimp zygotes of Penaeus (Litopenaeus) vannameiSemantic proximity Read