Article

Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b

1997-02-01

Abstract excerpt

Saturation mapping of a very small genomic region is indispensable for map-based cloning. We applied a method based on sub-cloning and the Southern-hybridization technique for generating RFLP markers directly from yeast artificial chromosomes (YACs). Two YACs overlapping each other and covering the locus of the rice blast resistance gene, Pi-b, were used to construct a plasmid sublibrary Rice-specific and single-c...

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Literature Corpus work: 1809f02a-a164-5b2c-81b6-bd04ebd93d46
DOI: 10.1007/s001220050396

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