Article

Cell-to-cell transmission of HSV1 in human keratinocytes in the absence of the major entry receptor, nectin1

2021-05-13

Abstract excerpt

Herpes simplex virus 1 (HSV1) infects the stratified epithelia of the epidermis, oral or genital mucosa, where the main cell type is the keratinocyte. Here we have used nTERT human keratinocytes to generate a CRISPR-Cas9 knockout (KO) of the primary candidate HSV1 receptor, nectin1, resulting in a cell line that is refractory to HSV1 entry. Nonetheless, a small population of KO cells was able to support infection,...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 15f9e6c0-9ba1-5a5d-9b90-60bb80013263
DOI: 10.1101/2021.05.13.443971

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Cell-to-cell transmission of HSV1 in human keratinocytes in the absence of the major entry receptor, nectin1DOI 10.1101/2021.05.13.443971Read

Herpes Simplex Virus 1 Enters Human Keratinocytes by a Nectin-1-Dependent, Rapid Plasma Membrane Fusion Pathway That Functions at Low TemperatureSemantic proximity Read

Ex Vivo Infection of Human Skin Models with Herpes Simplex Virus 1: Accessibility of the Receptor Nectin-1 during Formation or Impairment of Epidermal Barriers Is Restricted by Tight JunctionsSemantic proximity Read

Herpes simplex virus type 1 infection of human keratinocyte cellsSemantic proximity Read

Breaching the Barrier: Investigating Initial Herpes Simplex Viral Infection and Spread in Human Skin and MucosaSemantic proximity Read

Herpes Simplex Virus Assembly and Spread in murine skin after infection from the outsideSemantic proximity Read

Nectin-1-specific entry of herpes simplex virus 1 is sufficient for infection of the cornea and viral spread to the trigeminal ganglia.Semantic proximity Read

Release of HSV-1 Cell-Free Virions: Mechanisms, Regulation, and Likely Role in Human-Human TransmissionSemantic proximity Read

Cell-to-cell spread of wild-type herpes simplex virus type 1, but not of syncytial strains, is mediated by the immunoglobulin-like receptors that mediate virion entry, nectin1 (PRR1/HveC/HIgR) and nectin2 (PRR2/HveB).Semantic proximity Read

The herpes simplex virus JMP mutant enters receptor-negative J cells through a novel pathway independent of the known receptors nectin1, HveA, and nectin2.Semantic proximity Read

Nectin2alpha (PRR2alpha or HveB) and nectin2delta are low-efficiency mediators for entry of herpes simplex virus mutants carrying the Leu25Pro substitution in glycoprotein D.Semantic proximity Read

A human stem cell-derived neurosensory-epithelial circuitry on-a-chip to model herpes simplex virus reactivationSemantic proximity Read

Gene editing and elimination of latent herpes simplex virus in vivoSemantic proximity Read