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16S rDNA droplet digital PCR for monitoring bacterial DNAemia in bloodstream infections

13 Nov 2019

Abstract excerpt

Repeated quantitative measurement of bacterial DNA on whole blood has been shown to be a promising method for monitoring bloodstream infection (BSI) with selected bacterial species. To enable broad use of this method, we developed a quantitative droplet digital PCR (ddPCR) method for 16S rDNA. It was validated with species-specific ddPCRs for Staphylococcus aureus (nuc), Streptococcus pneumoniae (lytA), and...

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PMID: 31721817
DOI: 10.1371/journal.pone.0224656

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