Article

Polymerase chain reaction detection of Renibacterium salmoninarum in fish: Validation of a modified protocol

2009-02-01

Abstract excerpt

A PCR protocol (McIntosh et al., 1996. Appl. Environ. Microbiol. 62, 3929-2932), designed to detect the P57 gene of Renibacterium salmoninarum, causative agent of Bacterial Kidney Disease, was modified slightly by redesign of the forward primer and recommended conditions to prevent possible false positives observed when tested against pure cultures of Yersinia ruckeri. The modified PCR, in combination with an impr...

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Literature Corpus work: 0869c728-a322-5137-953f-26b16a0264f7
DOI: 10.1016/j.aquaculture.2008.10.031

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