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Detection of Yersinia ruckeri in rainbow trout blood by use of the polymerase chain reaction

1 Jan 2001

Abstract excerpt

We evaluated a polymerase chain reaction (PCR) method for detecting Yersinia ruckeri, the bacterial pathogen causing enteric redmouth disease (ERM), in blood of rainbow trout Oncorhynchus mykiss. Identification of the PCR product was confirmed by Southern blot hybridization with a 32P-labeled oligonucleotide probe matching a sequence within the small subunit ribosomal RNA gene of Y. ruckeri. Following a 1 h...

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PMID: 11253871
DOI: 10.3354/dao044029

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