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Rapid detection of porcine encephalomyocarditis virus (EMCV) by isothermal reverse transcription recombinase polymerase amplification assays

2022-03-31

Abstract excerpt

Reverse transcription recombinase polymerase amplification assays combined with the fluorescence detection platform (qRT-RPA) and lateral flow biosensor (LFB RT-RPA) were developed and validated for rapid detection of porcine EMCV. The primers and probes were designed based on the highly conserved region of 3D gene of porcine EMCV. The optimal reaction condition of qRT-RPA and LFB RT-RPA assay was at 42 °C for 20...

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Literature Corpus work
07114140-60eb-5de0-845a-18a14d359738
DOI
10.22541/au.164873416.62336819/v1
Open publication

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Rapid detection of porcine encephalomyocarditis virus (EMCV) by isothermal reverse transcription recombinase polymerase amplification assaysDOI 10.22541/au.164873416.62336819/v1
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