Article
P450c17 mutations R347H and R358Q selectively disrupt 17,20-lyase activity by disrupting interactions with P450 oxidoreductase and cytochrome b5.
Molecular endocrinology (Baltimore, Md.) - 1 Jan 1999
Geller D H, Auchus R J, Miller W L
Abstract excerpt
Cytochrome P450c17 catalyzes steroid 17alpha-hydroxylase and 17,20-lyase activities and hence is a key enzyme in the production of human glucocorticoids and sex steroids. These two activities are catalyzed in a single substrate-binding site but are regulated independently in human physiology. We...
Topics
- 17-alpha-Hydroxypregnenolone
- Animals
- COS Cells
- Cytochrome P-450 Enzyme System
- Cytochromes b5
- Humans
- Kinetics
- Microsomes
- Mutation
- Oxidoreductases
- Recombinant Proteins
- Steroid 17-alpha-Hydroxylase
- Structure-Activity Relationship
- Yeasts