Article
The C-terminal domain of the alpha subunit of Escherichia coli RNA polymerase is required for efficient rho-dependent transcription termination.
Journal of molecular biology - 18 Dec 1998
Kainz M, Gourse R L
Abstract excerpt
We screened a collection of single alanine residue substitution mutants spanning the entire C-terminal domain of the alpha subunit (alphaCTD) of Escherichia coli RNA polymerase (RNAP) for defects in rho-dependent transcription termination at lambdatR1 in vivo and in vitro, and thereby identified...
Topics
- Alanine
- Bacterial Proteins
- DNA-Directed RNA Polymerases
- Escherichia coli
- Escherichia coli Proteins
- Models, Molecular
- Mutation
- Peptide Elongation Factors
- Recombinant Proteins
- Rho Factor
- Structure-Activity Relationship
- Transcription Factors
- Transcription, Genetic
- Transcriptional Elongation Factors