Article
Measurement of resting cytosolic Ca2+ concentrations and Ca2+ store size in HEK-293 cells transfected with malignant hyperthermia or central core disease mutant Ca2+ release channels.
The Journal of biological chemistry - 8 Jan 1999
Tong J, McCarthy T V, MacLennan D H
Abstract excerpt
Malignant hyperthermia (MH) and central core disease (CCD) mutations were introduced into full-length rabbit Ca2+ release channel (RYR1) cDNA, which was then expressed transiently in HEK-293 cells. Resting Ca2+ concentrations were higher in HEK-293 cells expressing homotetrameric CCD mutant RyR1...
Topics
- Animals
- Caffeine
- Calcium
- Calcium Channels
- Calcium-Transporting ATPases
- Carbachol
- Cell Line
- Cytosol
- Endoplasmic Reticulum
- Humans
- Malignant Hyperthermia
- Mutation
- Myopathies, Nemaline
- Rabbits
- Ryanodine Receptor Calcium Release Channel
- Sarcoplasmic Reticulum
- Thapsigargin
- Transfection