Article
Use of polymerase chain reaction to simultaneously detect and type bovine viral diarrhoea viruses isolated from clinical specimens.
Revue scientifique et technique (International Office of Epizootics) - 1 Dec 1998
el-Kholy A A, Bolin S R, Ridpath J F, Arab R M, Abou-Zeid A A, Hammam H M, Platt K B
Abstract excerpt
The techniques of indirect immunofluorescence (IF), immuno-peroxidase (IP) staining and the one-step reverse transcriptase polymerase chain reaction (RT-PCR) were compared for detection of 102 isolates of bovine viral diarrhoea virus (BVDV) in infected cell cultures. The BVDV was obtained from bovine clinical specimens, including sera, buffy coats and tissues, submitted from farms located in the States of Iowa...
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