Article
Use of polymerase chain reaction to simultaneously detect and type bovine viral diarrhoea viruses isolated from clinical specimens.
Revue scientifique et technique (International Office of Epizootics) - 1 Dec 1998
el-Kholy A A, Bolin S R, Ridpath J F, Arab R M, Abou-Zeid A A, Hammam H M, Platt K B
Abstract excerpt
The techniques of indirect immunofluorescence (IF), immuno-peroxidase (IP) staining and the one-step reverse transcriptase polymerase chain reaction (RT-PCR) were compared for detection of 102 isolates of bovine viral diarrhoea virus (BVDV) in infected cell cultures. The BVDV was obtained from bo...
Topics
- Animals
- Antigens, Viral
- Bovine Virus Diarrhea-Mucosal Disease
- Cattle
- Cells, Cultured
- Diarrhea Viruses, Bovine Viral
- Electrophoresis, Agar Gel
- Female
- Fluorescent Antibody Technique, Indirect
- Genotype
- Immunoenzyme Techniques
- Male
- RNA, Viral
- Reverse Transcriptase Polymerase Chain Reaction