Combination of Reverse Transcription Real-Time Polymerase Chain Reaction and Antigen Capture Enzyme-Linked Immunosorbent Assay for the Detection of Animals Persistently Infected with Bovine Viral Diarrhea Virus

Abstract excerpt

Bovine viral diarrhea virus (BVDV) is an economically important pathogen of cattle. A successful control program requires early detection and removal of persistently infected (PI) animals. The objective of the current study was to develop, validate, and apply a cost-effective testing scheme for the detection of BVDV PI animals in exposed herds. Pooled samples were screened by using a real-time reverse...

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