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Article

Detection and genotyping of bovine diarrhea virus by reverse transcription-polymerase chain amplification of the 5' untranslated region.

Veterinary microbiology - 1 Jan 1999

Letellier C, Kerkhofs P, Wellemans G, Vanopdenbosch E

Abstract excerpt

A reverse-transcription polymerase chain reaction (RT-PCR) was developed to differentiate the bovine diarrhea virus (BVDV) from other pestiviruses, and to determine the genotype of the BVDV isolates. For this purpose, primer pairs were selected in the 5' untranslated region (5'UTR). The primers BE and B2 were located in highly conserved regions and were pestivirus-specific. Two primer pairs named B3B4 and B5B6...

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Identifiers

PMID: 10028170
DOI: 10.1016/s0378-1135(98)00267-3
PMCID: PMC7117503

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Source receipt: pubmed, retrieved 7 Sept 2026.

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