Article
A universal 'one-tube' RT-PCR protocol for amplifying isolates of bovine viral diarrhoea virus.
Veterinary research communications - 1 Nov 2000
Pfeffer M, Freyburg M V, Kaaden O R, Beer M
Abstract excerpt
The increase in the knowledge of the genetic variability of BVDV and the identification of some of the genetic determinants of its pathogenicity require robust and practical tools for rapid molecular characterization of the various genotypes of this virus. This study was undertaken to develop a standard protocol for RT-PCR that allows the amplification of various parts of the genome of BVDV without the need for...
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