Article
The Escherichia coli cmlA gene encodes the multidrug efflux pump Cmr/MdfA and is responsible for isopropyl-beta-D-thiogalactopyranoside exclusion and spectinomycin sensitivity.
Journal of bacteriology - 1 Nov 1998
Bohn C, Bouloc P
Abstract excerpt
Expression of cloned genes from isopropyl-beta-D-thiogalactopyranoside (IPTG)-regulated promoters is lowered when the Escherichia coli CmlA/Cmr/MdfA efflux pump is overexpressed, probably due to IPTG exclusion from the cytoplasm. The previously reported cmlA1 mutation confers a similar phenotype....
Topics
- Alleles
- Bacterial Proteins
- Carrier Proteins
- Drug Resistance, Microbial
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression
- Isopropyl Thiogalactoside
- Membrane Proteins
- Membrane Transport Proteins
- Mutagenesis
- Plasmids
- Spectinomycin
- beta-Galactosidase