Article
Expression of glycosylated recombinant human myelin-associated glycoprotein on a neuroblastoma cell line and its reactivity with HNK-1 but not human anti-MAG antibodies.
Neuroscience letters - 1 May 1998
Spagnol G, Doneda P, Cavanna B, Sgarzi M, Manfredini E, Allaria S, Scarlato G, Nobile-Orazio E
Abstract excerpt
A correctly glycosylated myelin-associated glycoprotein (MAG) must express the carbohydrate epitope HNK-1, which is the target antigen for IgM antibodies in some patients with neuropathy. We transfected a human MAG cDNA clone into the neuroblastoma cell line SK-N-SH and verified by immunoblot the...
Topics
- Antibodies
- CD57 Antigens
- DNA, Complementary
- Epitopes
- Fluorescent Antibody Technique, Indirect
- Gene Expression
- Glycosylation
- Humans
- Immunoblotting
- Myelin-Associated Glycoprotein
- Neuroblastoma
- Neurons
- Phenotype
- Recombinant Proteins
- Schwann Cells