Article
Cloning and sequencing of the Candida albicans homologue of SRB1/PSA1/VIG9, the essential gene encoding GDP-mannose pyrophosphorylase in Saccharomyces cerevisiae.
Microbiology (Reading, England) - 1 Sept 1998
Warit Saradee, Walmsley Richard M, Stateva Lubomira I
Abstract excerpt
Two genomic fragments have been isolated from Candida albicans which strongly hybridize to SRB1/PSA1/VIG9, an essential gene which encodes GDP-mannose pyrophosphorylase in Saccharomyces cerevisiae. A common 2.5 kb Xbal-Pstl fragment has been identified, which Southern analysis suggests is most li...
Topics
- Amino Acid Sequence
- Base Sequence
- Candida albicans
- Cloning, Molecular
- DNA Primers
- Escherichia coli
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Nucleotidyltransferases
- Open Reading Frames
- Phenotype
- Polymerase Chain Reaction
- Saccharomyces cerevisiae
- Sequence Homology, Amino Acid
- Species Specificity